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anti fis 1 rabbit polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti fis 1 rabbit polyclonal antibody
    Anti Fis 1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+fis1/FIS1+Polyclonal+antibody/pmc12794071-323-12-18
    Average 96 stars, based on 345 article reviews
    anti fis 1 rabbit polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The Synthetic Cannabinoid ADB-FUBINACA Disrupts Mitochondrial Morphology and Dynamics during Neuronal Differentiation of NG108-15 Cells.
    Article Snippet: .. After three 10-min washes with TPBS, the membranes were incubated overnight at 4° C with primary antibodies: mouse anti-Drp1 (1:200, sc-271583), mouse anti-Mfn1 (1:100, sc-166644), mouse anti-Mfn2 (1:400, sc-100560), mouse anti-Miro1 (1:200, sc-398520), mouse p-Tau antibody (1:200, sc-32275), mouse Tau (1:200, sc-21796, Santa Cruz Biotechnology, CA, USA), mouse Opa1 (1:1000, Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-Phospho-DRP1 (Ser616) (1:500, Invitrogen PA564,821), rabbit anti-MiD51 (1:1000, Invitrogen PA5-99,970) and rabbit anti-Fis1 (1:2000, Proteintech, IL, USA). .. Samples were additionally probed with mouse anti-β-actin antibody (1:4000, Sigma-Aldrich, St Louis, MO, USA) to normalize the data obtained.

    Article Title: Mitofusin 2 is required for preventing deoxynivalenol-induced porcine intestinal epithelial cell damage
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes were incubated overnight with the following primary antibodies: rabbit anti-Drp1 (1:1,000, # Ab154879 , Abcam), rabbit anti-Fis1 (1:1,000, #10956-1-AP, Proteintech), rabbit anti-Opa1 (1:1,000, #NB110-55290ss, Novus), rabbit anti-Mfn1 (1:1,000, #PA5-44826, Invitrogen), mouse anti-Mfn2 (1:1,000, #ab56889, Abcam), and mouse anti-β-actin (1:2,000, #4970s, CST). ..

    Article Title: The Synthetic Cannabinoid ADB-FUBINACA Disrupts Mitochondrial Morphology and Dynamics during Neuronal Differentiation of NG108-15 Cells
    Article Snippet: .. After three 10-min washes with TPBS, the membranes were incubated overnight at 4° C with primary antibodies: mouse anti-Drp1 (1:200, sc-271583), mouse anti-Mfn1 (1:100, sc-166644), mouse anti-Mfn2 (1:400, sc-100560), mouse anti-Miro1 (1:200, sc-398520), mouse p-Tau antibody (1:200, sc-32275), mouse Tau (1:200, sc-21796, Santa Cruz Biotechnology, CA, USA), mouse Opa1 (1:1000, Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-Phospho-DRP1 (Ser616) (1:500, Invitrogen PA5-64,821), rabbit anti-MiD51 (1:1000, Invitrogen PA5-99,970) and rabbit anti-Fis1 (1:2000, Proteintech, IL, USA). .. Samples were additionally probed with mouse anti-β-actin antibody (1:4000, Sigma-Aldrich, St Louis, MO, USA) to normalize the data obtained.

    Article Title: Mitofusin 2 is required for preventing deoxynivalenol-induced porcine intestinal epithelial cell damage.
    Article Snippet: .. After blocking with 5% nonfat milk, the membranes were incubated overnight with the following primary antibodies: rabbit antiDrp1 (1:1,000, #Ab154879, Abcam), rabbit anti-Fis1 (1:1,000, #10956-1-AP, Proteintech), rabbit anti-Opa1 (1:1,000, #NB110-55290ss, Novus), rabbit anti-Mfn1 (1:1,000, #PA5-44826, Invitrogen), mouse anti-Mfn2 (1:1,000, #ab56889, Abcam), and mouse anti-β-actin (1:2,000, #4970s, CST). ..

    Article Title: Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone Regeneration.
    Article Snippet: .. After blocking, the proteins were incubated with rabbit anti-Fis1 (10956-1-AP, Proteintech, Rosemont, IL, USA), rabbit anti-Mff (17090-1-AP, Proteintech), and rabbit anti-p-Drp1 (12957-1-AP, Proteintech) at 4 °C overnight, followed by incubation with a goat anti-rabbit IgG (Yeasen, Shanghai, China) secondary antibody. β- tubulin (#2146, Cell Signaling, Portland, OR, USA) and COX IV (#4844, Cell Signaling) were used as the internal control. ..

    Blocking Assay:

    Article Title: Mitofusin 2 is required for preventing deoxynivalenol-induced porcine intestinal epithelial cell damage
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes were incubated overnight with the following primary antibodies: rabbit anti-Drp1 (1:1,000, # Ab154879 , Abcam), rabbit anti-Fis1 (1:1,000, #10956-1-AP, Proteintech), rabbit anti-Opa1 (1:1,000, #NB110-55290ss, Novus), rabbit anti-Mfn1 (1:1,000, #PA5-44826, Invitrogen), mouse anti-Mfn2 (1:1,000, #ab56889, Abcam), and mouse anti-β-actin (1:2,000, #4970s, CST). ..

    Article Title: Mitofusin 2 is required for preventing deoxynivalenol-induced porcine intestinal epithelial cell damage.
    Article Snippet: .. After blocking with 5% nonfat milk, the membranes were incubated overnight with the following primary antibodies: rabbit antiDrp1 (1:1,000, #Ab154879, Abcam), rabbit anti-Fis1 (1:1,000, #10956-1-AP, Proteintech), rabbit anti-Opa1 (1:1,000, #NB110-55290ss, Novus), rabbit anti-Mfn1 (1:1,000, #PA5-44826, Invitrogen), mouse anti-Mfn2 (1:1,000, #ab56889, Abcam), and mouse anti-β-actin (1:2,000, #4970s, CST). ..

    Article Title: Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone Regeneration.
    Article Snippet: .. After blocking, the proteins were incubated with rabbit anti-Fis1 (10956-1-AP, Proteintech, Rosemont, IL, USA), rabbit anti-Mff (17090-1-AP, Proteintech), and rabbit anti-p-Drp1 (12957-1-AP, Proteintech) at 4 °C overnight, followed by incubation with a goat anti-rabbit IgG (Yeasen, Shanghai, China) secondary antibody. β- tubulin (#2146, Cell Signaling, Portland, OR, USA) and COX IV (#4844, Cell Signaling) were used as the internal control. ..

    Control:

    Article Title: Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone Regeneration.
    Article Snippet: .. After blocking, the proteins were incubated with rabbit anti-Fis1 (10956-1-AP, Proteintech, Rosemont, IL, USA), rabbit anti-Mff (17090-1-AP, Proteintech), and rabbit anti-p-Drp1 (12957-1-AP, Proteintech) at 4 °C overnight, followed by incubation with a goat anti-rabbit IgG (Yeasen, Shanghai, China) secondary antibody. β- tubulin (#2146, Cell Signaling, Portland, OR, USA) and COX IV (#4844, Cell Signaling) were used as the internal control. ..

    Immunofluorescence:

    Article Title: Rab14 promotes Parkin mediated mitophagy
    Article Snippet: Mitochondrial degradation by mitophagy is essential to maintain cell metabolism; dysregulation can result in the accumulation of damaged mitochondria.. While the Rab family of small GTPase proteins are involved with vesicular trafficking in the endocytic and biosynthetic pathways, Rab-GTPases also have a role in mitochondrial integrity.. However, a role for Rab14, a trans-Golgi network (TGN)-endosomal Rab-GTPase in mitophagy has not been described.

    Western Blot:

    Article Title: Rab14 promotes Parkin mediated mitophagy
    Article Snippet: Mitochondrial degradation by mitophagy is essential to maintain cell metabolism; dysregulation can result in the accumulation of damaged mitochondria.. While the Rab family of small GTPase proteins are involved with vesicular trafficking in the endocytic and biosynthetic pathways, Rab-GTPases also have a role in mitochondrial integrity.. However, a role for Rab14, a trans-Golgi network (TGN)-endosomal Rab-GTPase in mitophagy has not been described.



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    Group comparison of mitochondrial Drp1 ( A ) and <t>Fis1</t> ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .
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    Proteintech rabbit anti fis1
    Group comparison of mitochondrial Drp1 ( A ) and <t>Fis1</t> ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .
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    Image Search Results


    Group comparison of mitochondrial Drp1 ( A ) and Fis1 ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .

    Journal: Cells

    Article Title: Cys340Ser Mutation Abolishing S-Nitrosylation Drives GRK2 Mitochondrial Localization and Dysfunction

    doi: 10.3390/cells15050458

    Figure Lengend Snippet: Group comparison of mitochondrial Drp1 ( A ) and Fis1 ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .

    Article Snippet: The primary antibodies used were as follows: GRK2 (13990-1-AP; ProteinTech, Rosemont, IL, USA), GRK2 (05-465; Sigma Aldrich, St. Louis, MO, USA), Drp1 (8570; Cell Signaling Technology, Danvers, MA, USA), Fis1 (32525; Cell Signaling Technology, Danvers, MA, USA), Mfn1 (14739; Cell Signaling Technology, Danvers, MA, USA), Mfn2 (11925; Cell Signaling Technology, Danvers, MA, USA), Opa1 (80471; Cell Signaling Technology), Pink (6946; Cell Signaling Technology, Danvers, MA, USA), Parkin (4211; Cell Signaling Technology, Danvers, MA, USA), LC3 (3868; Cell Signaling Technology, Danvers, MA, USA), Tom20 (42406; Cell Signaling Technology, Danvers, MA, USA), VDAC1 (sc-390996; Santa Cruz Biotechnology, Dallas, TX, USA), and GAPDH (sc-32233; Santa Cruz Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Comparison, Western Blot, Infection, Control, Marker, Software, Molecular Weight